Robust, fiducial-free drift correction for super-resolution imaging

Robust, fiducial-free drift correction for super-resolution imaging

Blog Article

Abstract We describe a robust, fiducial-free method of drift correction for use in single molecule localization-based super-resolution wella color touch 77 45 methods.The method combines periodic 3D registration of the sample using brightfield images with a fast post-processing algorithm that ilootpaperie corrects residual registration errors and drift between registration events.The method is robust to low numbers of collected localizations, requires no specialized hardware, and provides stability and drift correction for an indefinite time period.